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Isolation, Purification and Structural Characterization of a Polysaccharide from Rheum Palmatum L.

Received: 14 February 2017     Accepted: 22 March 2017     Published: 14 April 2017
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Abstract

Rhubarb is a widely used Traditional Chinese Medicine in treating various diseases for thousands of years, and polysaccharide is one of the bioactive constituents in this herb. However, study of the polysaccharide is very limited, especially structural characterization. In the present study, a polysaccharide from Rheum palmatum L. (PR) was isolated and puried. Based on UV, IR, GC, GC-MS, 1H NMR and 13C NMR analyses, the structure of PR was characterized. Its molecular weight was measured to be 1.21 × 104 Da. The results of PR structural analysis showed that four 1→4 galactose were linked with one 1→ 3, 6 galactose and one 1→6 glucose, and each repeating unit had a branched chain consisting of a α-1→ xylose, which was linked with the C-6 of galactose backbone chain.

Published in Journal of Food and Nutrition Sciences (Volume 5, Issue 3)
DOI 10.11648/j.jfns.20170503.13
Page(s) 73-78
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2017. Published by Science Publishing Group

Keywords

Rheum Palmatum L., Polysaccharide, Isolation, Purification, Structural Characterization

References
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  • APA Style

    Jinhua Li, Sihao Zheng, Weiguang Ren, Labin Wu, Linfang Huang. (2017). Isolation, Purification and Structural Characterization of a Polysaccharide from Rheum Palmatum L.. Journal of Food and Nutrition Sciences, 5(3), 73-78. https://doi.org/10.11648/j.jfns.20170503.13

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    ACS Style

    Jinhua Li; Sihao Zheng; Weiguang Ren; Labin Wu; Linfang Huang. Isolation, Purification and Structural Characterization of a Polysaccharide from Rheum Palmatum L.. J. Food Nutr. Sci. 2017, 5(3), 73-78. doi: 10.11648/j.jfns.20170503.13

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    AMA Style

    Jinhua Li, Sihao Zheng, Weiguang Ren, Labin Wu, Linfang Huang. Isolation, Purification and Structural Characterization of a Polysaccharide from Rheum Palmatum L.. J Food Nutr Sci. 2017;5(3):73-78. doi: 10.11648/j.jfns.20170503.13

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  • @article{10.11648/j.jfns.20170503.13,
      author = {Jinhua Li and Sihao Zheng and Weiguang Ren and Labin Wu and Linfang Huang},
      title = {Isolation, Purification and Structural Characterization of a Polysaccharide from Rheum Palmatum L.},
      journal = {Journal of Food and Nutrition Sciences},
      volume = {5},
      number = {3},
      pages = {73-78},
      doi = {10.11648/j.jfns.20170503.13},
      url = {https://doi.org/10.11648/j.jfns.20170503.13},
      eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.jfns.20170503.13},
      abstract = {Rhubarb is a widely used Traditional Chinese Medicine in treating various diseases for thousands of years, and polysaccharide is one of the bioactive constituents in this herb. However, study of the polysaccharide is very limited, especially structural characterization. In the present study, a polysaccharide from Rheum palmatum L. (PR) was isolated and puried. Based on UV, IR, GC, GC-MS, 1H NMR and 13C NMR analyses, the structure of PR was characterized. Its molecular weight was measured to be 1.21 × 104 Da. The results of PR structural analysis showed that four 1→4 galactose were linked with one 1→ 3, 6 galactose and one 1→6 glucose, and each repeating unit had a branched chain consisting of a α-1→ xylose, which was linked with the C-6 of galactose backbone chain.},
     year = {2017}
    }
    

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  • TY  - JOUR
    T1  - Isolation, Purification and Structural Characterization of a Polysaccharide from Rheum Palmatum L.
    AU  - Jinhua Li
    AU  - Sihao Zheng
    AU  - Weiguang Ren
    AU  - Labin Wu
    AU  - Linfang Huang
    Y1  - 2017/04/14
    PY  - 2017
    N1  - https://doi.org/10.11648/j.jfns.20170503.13
    DO  - 10.11648/j.jfns.20170503.13
    T2  - Journal of Food and Nutrition Sciences
    JF  - Journal of Food and Nutrition Sciences
    JO  - Journal of Food and Nutrition Sciences
    SP  - 73
    EP  - 78
    PB  - Science Publishing Group
    SN  - 2330-7293
    UR  - https://doi.org/10.11648/j.jfns.20170503.13
    AB  - Rhubarb is a widely used Traditional Chinese Medicine in treating various diseases for thousands of years, and polysaccharide is one of the bioactive constituents in this herb. However, study of the polysaccharide is very limited, especially structural characterization. In the present study, a polysaccharide from Rheum palmatum L. (PR) was isolated and puried. Based on UV, IR, GC, GC-MS, 1H NMR and 13C NMR analyses, the structure of PR was characterized. Its molecular weight was measured to be 1.21 × 104 Da. The results of PR structural analysis showed that four 1→4 galactose were linked with one 1→ 3, 6 galactose and one 1→6 glucose, and each repeating unit had a branched chain consisting of a α-1→ xylose, which was linked with the C-6 of galactose backbone chain.
    VL  - 5
    IS  - 3
    ER  - 

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Author Information
  • Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, China

  • Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, China

  • Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, China

  • Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, China

  • Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, China

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