| Peer-Reviewed

Milk Traces Detection in Cookies and Extruded Products Analyzed with Elisa Kits

Received: 23 September 2016     Accepted: 7 October 2016     Published: 4 January 2017
Views:       Downloads:
Abstract

Detection of milk traces was evaluated in cookies and extruded products model systems (MS) and in commercial products declaring milk presence through either the statement: “Contains…” or precautionary phrases. The aim of the study was to evaluate the performance of two different commercial ELISA kits for this purpose. Six cookies and extruded products model systems and two different batches of eight different types of commercial cookies and two types of extruded products were analyzed. The two different commercial ELISA kits used were R-Biopharm and Veratox-Neogen. In the cookies MS both kits had the same sensitivity while in the extruded products MS, the R- Biopharm kit had higher sensitivity than the Veratox-Neogen one. In the commercial samples only the snack declaring “Contains milk derivatives” showed detectable values of milk proteins. Even though all the remaining products included precautionary phrases on the label, none of them exceeded the quantification limit. The detection and quantification of the allergen will depend on the utilized kit and the treatment to which the ingredients have been submitted. Therefore, the performance of every kit should be studied for every allergen and every particular food matrix. Regarding commercial products, the analysis of more samples coming from different batches would be necessary in order to corroborate these results and verify the correct use of precautionary phrases.

Published in Journal of Food and Nutrition Sciences (Volume 4, Issue 6)
DOI 10.11648/j.jfns.20160406.16
Page(s) 175-179
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2017. Published by Science Publishing Group

Keywords

Allergens, Milk, Cookies, Extruded Products, ELISA

References
[1] Chapman J, Bernstein L, Lee R, Oppenheimer J. 2006. Food allergy: a practice parameter. Annals of Allergy, Asthma & Immunology. 96 (3 Suppl 2): S1-S68.
[2] Díaz Amigo C. 2010. Towards a Comprehensive Validation of ELISA Kits or Food Allergens. Case2-Milk. Food Analytical Methods. 3: 351-356.
[3] Cellerino K, Binaghi MJ, Cagnasso CE, Docena G, López LB. 2014. Milk protein detection in raw and cooked meat products using immunochemical methods. Journal of Food and Nutrition Sciences. 2(5): 236-242.
[4] Act N° 111 CONAL. 2016. http://www.conal.gob.ar/actas/Acta_111.pdf Access: 06/ 09/2016.
[5] López L, Binaghi M, Greco C, Mambrín M, Valencia M. 2011. Meat species identification in cooked meat products: using SDS-PAGE as screening method. Revista Chilena de Nutrición. 38 (2): 187-196.
[6] RIDASCREEN® Fast Milk protein (Art. No.: R4652). R-Biopharm. 2016. Available: http://www.R-Biopharm.com/products/food-feed-analysis/allergens/milk/item/ridascreenfast-milk. Access: September 2016.
[7] Veratox® Total Milk (Code: 8470). Neogen, 2016. Available: http://foodsafety.neogen.com/en/veratox-total-milk Access: September 2016.
[8] Díaz Amigo C, Popping B. 2010. Analytical Testing as a Tool for the Enforcement of Future Regulatory Thresholds for Food Allergens. Journal of AOAC International. 93 (2): 434-441.
[9] Parker C, Khuda S, Pereira M, Ross M, Fu T, Fan X, Wu Y, Williams K, DeVries J, Pulvermacher B, Bedford B, Zhang X and Jackson L. 2015. Multi-allergen Quantitation and the Impact of Thermal Treatment in Industry-Processed Baked Goods by ELISA and Liquid Chromatography-Tandem Mass Spectrometry. Journal of Agricultural and Food Chemistry. 63: 10669−10680.
[10] Popping B, Diaz-Amigo C, Hoenicke K. 2010. Chapter 17 in Molecular Biological and immunological techniques and applications for food chemists. John Wiley & Sons, Inc. Canada.
[11] Besler M., Steinhart H., Paschke A. 2001. Stability of food allergens and allergenicity of processed food. Journal of chromatography B: Biomedical Sciences and Applications. 756 (1-2): 207-228.
[12] Díaz Amigo C. 2010. Towards a Comprehensive Validation of ELISA Kits for Food Allergens. Case 1- Egg. Food Analytical Methods. 3: 344-350.
[13] Sampson MA, Munoz-Furlong A, & Sicherer SH. 2006. Risktaking and coping strategies of adolescents and young adults with food allergy. Journal of Allergy and Clinical Immunology. 117: 1440-1445.
[14] Ward R, Crevel R, Bell I, Khandke N, Ramsay C and Paine S. 2010. A vision for allergen management best practice in the food industry. Trends in Food Science & Technology 21: 619-625.
Cite This Article
  • APA Style

    Binaghi Maria Julieta, Greco Carola Beatriz, Martín Maria Eugenia, Drago Silvina Rosa, Ronayne de Ferrer Patricia Ana, et al. (2017). Milk Traces Detection in Cookies and Extruded Products Analyzed with Elisa Kits. Journal of Food and Nutrition Sciences, 4(6), 175-179. https://doi.org/10.11648/j.jfns.20160406.16

    Copy | Download

    ACS Style

    Binaghi Maria Julieta; Greco Carola Beatriz; Martín Maria Eugenia; Drago Silvina Rosa; Ronayne de Ferrer Patricia Ana, et al. Milk Traces Detection in Cookies and Extruded Products Analyzed with Elisa Kits. J. Food Nutr. Sci. 2017, 4(6), 175-179. doi: 10.11648/j.jfns.20160406.16

    Copy | Download

    AMA Style

    Binaghi Maria Julieta, Greco Carola Beatriz, Martín Maria Eugenia, Drago Silvina Rosa, Ronayne de Ferrer Patricia Ana, et al. Milk Traces Detection in Cookies and Extruded Products Analyzed with Elisa Kits. J Food Nutr Sci. 2017;4(6):175-179. doi: 10.11648/j.jfns.20160406.16

    Copy | Download

  • @article{10.11648/j.jfns.20160406.16,
      author = {Binaghi Maria Julieta and Greco Carola Beatriz and Martín Maria Eugenia and Drago Silvina Rosa and Ronayne de Ferrer Patricia Ana and López Laura Beatriz},
      title = {Milk Traces Detection in Cookies and Extruded Products Analyzed with Elisa Kits},
      journal = {Journal of Food and Nutrition Sciences},
      volume = {4},
      number = {6},
      pages = {175-179},
      doi = {10.11648/j.jfns.20160406.16},
      url = {https://doi.org/10.11648/j.jfns.20160406.16},
      eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.jfns.20160406.16},
      abstract = {Detection of milk traces was evaluated in cookies and extruded products model systems (MS) and in commercial products declaring milk presence through either the statement: “Contains…” or precautionary phrases. The aim of the study was to evaluate the performance of two different commercial ELISA kits for this purpose. Six cookies and extruded products model systems and two different batches of eight different types of commercial cookies and two types of extruded products were analyzed. The two different commercial ELISA kits used were R-Biopharm and Veratox-Neogen. In the cookies MS both kits had the same sensitivity while in the extruded products MS, the R- Biopharm kit had higher sensitivity than the Veratox-Neogen one. In the commercial samples only the snack declaring “Contains milk derivatives” showed detectable values of milk proteins. Even though all the remaining products included precautionary phrases on the label, none of them exceeded the quantification limit. The detection and quantification of the allergen will depend on the utilized kit and the treatment to which the ingredients have been submitted. Therefore, the performance of every kit should be studied for every allergen and every particular food matrix. Regarding commercial products, the analysis of more samples coming from different batches would be necessary in order to corroborate these results and verify the correct use of precautionary phrases.},
     year = {2017}
    }
    

    Copy | Download

  • TY  - JOUR
    T1  - Milk Traces Detection in Cookies and Extruded Products Analyzed with Elisa Kits
    AU  - Binaghi Maria Julieta
    AU  - Greco Carola Beatriz
    AU  - Martín Maria Eugenia
    AU  - Drago Silvina Rosa
    AU  - Ronayne de Ferrer Patricia Ana
    AU  - López Laura Beatriz
    Y1  - 2017/01/04
    PY  - 2017
    N1  - https://doi.org/10.11648/j.jfns.20160406.16
    DO  - 10.11648/j.jfns.20160406.16
    T2  - Journal of Food and Nutrition Sciences
    JF  - Journal of Food and Nutrition Sciences
    JO  - Journal of Food and Nutrition Sciences
    SP  - 175
    EP  - 179
    PB  - Science Publishing Group
    SN  - 2330-7293
    UR  - https://doi.org/10.11648/j.jfns.20160406.16
    AB  - Detection of milk traces was evaluated in cookies and extruded products model systems (MS) and in commercial products declaring milk presence through either the statement: “Contains…” or precautionary phrases. The aim of the study was to evaluate the performance of two different commercial ELISA kits for this purpose. Six cookies and extruded products model systems and two different batches of eight different types of commercial cookies and two types of extruded products were analyzed. The two different commercial ELISA kits used were R-Biopharm and Veratox-Neogen. In the cookies MS both kits had the same sensitivity while in the extruded products MS, the R- Biopharm kit had higher sensitivity than the Veratox-Neogen one. In the commercial samples only the snack declaring “Contains milk derivatives” showed detectable values of milk proteins. Even though all the remaining products included precautionary phrases on the label, none of them exceeded the quantification limit. The detection and quantification of the allergen will depend on the utilized kit and the treatment to which the ingredients have been submitted. Therefore, the performance of every kit should be studied for every allergen and every particular food matrix. Regarding commercial products, the analysis of more samples coming from different batches would be necessary in order to corroborate these results and verify the correct use of precautionary phrases.
    VL  - 4
    IS  - 6
    ER  - 

    Copy | Download

Author Information
  • Faculty of Pharmacy and Biochemistry, Universidad de Buenos Aires – University of Buenos Aires, Buenos Aires, Argentina

  • Faculty of Pharmacy and Biochemistry, Universidad de Buenos Aires – University of Buenos Aires, Buenos Aires, Argentina

  • Faculty of Pharmacy and Biochemistry, Universidad de Buenos Aires – University of Buenos Aires, Buenos Aires, Argentina

  • Food Technology Institute, University Nacional of Litoral, Santa Fe, Argentina

  • Faculty of Pharmacy and Biochemistry, Universidad de Buenos Aires – University of Buenos Aires, Buenos Aires, Argentina

  • Faculty of Pharmacy and Biochemistry, Universidad de Buenos Aires – University of Buenos Aires, Buenos Aires, Argentina

  • Sections