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Improvement in High-fat Diet-induced Obesity and Insulin Resistance upon Uptake of PS-B1, a Fermented Product Prepared from Soy Flour Using Lactic Acid Bacteria

Received: 3 June 2020     Accepted: 19 June 2020     Published: 4 July 2020
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Abstract

PS-B1 is a fermented product prepared from soy flour using lactic acid bacteria. Over a 10-week period, C57BL/6J mice were reared under laboratory conditions on a normal diet (control, n=5), high-fat diet (HF, n=5), or high-fat diet supplemented with 4% PS-B1 (HF-P, n=6). After 10 weeks, the change in weight gain, intestinal and epididymal fat accumulation, serum and liver biochemical parameters, and gene expression in the mice was investigated. HF diet-induced weight gain and increase in intestinal and epididymal fat accumulation were lower in mice fed with HF-P diet than in mice fed with HF diet, suggesting that PS-B1 prevented HF diet-induced obesity in HF-P mice. Furthermore, the levels of liver lipids (triglycerides, TG; non-esterified fatty acid, NEFA; total cholesterol, TC), serum TC, serum glucose, and serum insulin were significantly increased in the HF group than those in control mice. In HF-P mice, neither serum TC nor serum glucose levels were reduced. In contrast, the levels of liver lipids and serum insulin were lower in HF-P mice than in HF mice, suggesting that PS-B1 reduced these parameters in HF-P mice. The homeostatic model assessment of insulin resistance (HOMA-IR) value, which was calculated from the serum glucose and insulin levels, was 21.5 ± 4.2 in the HF mice. However, the HOMA-IR (8.2 ± 0.2) values were significantly decreased in the HF-P mice, suggesting that PS-B1 improves insulin resistance. Additionally, we compared the expression levels of stearoyl-CoA desaturase-1 (Scd1) in the liver. Quantitative RT-PCR showed increased expression of Scd1 in HF mice compared to that in control mice. Furthermore, ingestion of PS-B1 led to reduced expression of Scd1 mRNA in HF-P mice, implying that PS-B1 is effective in reducing the expression of the gene encoding SCD1. These results suggest that the anti-obesity effect of PS-B1 and improvement in fat accumulation upon PS-B1 uptake may be due to improvement in insulin resistance and reduction in the expression level of Scd1.

Published in Journal of Food and Nutrition Sciences (Volume 8, Issue 4)
DOI 10.11648/j.jfns.20200804.12
Page(s) 81-88
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2020. Published by Science Publishing Group

Keywords

Lactic Acid Bacteria, Soy Flour, Biogenics, Lipid Metabolism, Obesity Prevention

References
[1] Fuller R., “Probiotics in man and animals,” J. appl. bacteriol., 66, 365-378, 1989.
[2] Wong V. W., Wong G. L., Chim A. M., Chu W. C., Yeung D. K., Li K. C., and Chan H. L., “Treatment of nonalcoholic steatohepatitis with probiotics. A proof-of-concept study,” Ann Hepatol., 12, 256-262, 2013.
[3] Sun J., and Buys N. J., “Glucose- and glycaemic factor-lowering effects of probiotics on diabetes: a meta-analysis of randomised placebo-controlled trials,” Br. J. Nutr., 115, 1167-1177, 2016.
[4] Huang R., Wang K., and Hu J., “Effect of probiotics on depression: A systematic review and meta-analysis of randomized controlled trials,” Nutrients., 8, 483, 2016, doi: 10.3390/nu8080483.
[5] Takahashi T., Masuda Y., Yoshida K., and Mizuno M., “Development of fermented rice drink using lactic acid bacteria isolated from kimoto,” Seibutsu-kogaku., 94, 63-69, 2016.
[6] Aoi W., Iwasa M., and Abe R., “Investigation of fermented milk-derived biogenics that improve exercise-induced glucose metabolism in middle-aged to elderly people,” Descente sports science., 38, 164-171, 2017.
[7] Odashiro K., Fukata M., Saito K., Wakana C., Maruyama T., Sasuga Y., Fukui M., and Fujino T., “The effects of lactic acid bacteria-fermented soymilk extract on patients with colonic polyps: a randomized, double-blind, placebo-controlled pilot trial,” J. Integr. Stud. Diet. Habits., 25, 20-25, 2014.
[8] Mitsuoka T., “Significance of dietary modulation of intestinal flora and intestinal environment,” Bioscience Microflora., 19, 15-25, 2000.
[9] Nodake Y., Miura R., Ryoya H., Momii R., Toda S., and Sakakibara R., “Improvement of lipid metabolism and ovalbumin-induced type Ⅰ allergy by use of soybean milk fermented by 16 indigenous lactic acid bacteria,” J. Food Nutr. Sci., 4, 113-119, 2016, doi: 10.11648/j.jfns.20160404.17.
[10] Yamaguchi K., Matsumoto S., Fuchigami T., Tsuchida M., Honda H., and Sakakibara R., “Effect of PS-B1 (a fermented product cultured from soy flour using lactic acid bacteria) on defecation and skin condition,” J. Integr. Stud. Diet. Habits., 30, 111-122, 2020.
[11] Nodake Y., Ogasawara M., Honda H., Fukasawa M., and Sakakibara R., “Characterization and preliminary purification of the anti-cancer substance (s) in the fermented products cultivated from soybean milk using lactic acid bacteria,” Saito Ho-on kai Mus. Nat. Hist., Res. Bull., 73, 17-21, 2008.
[12] Nodake Y., Fukasawa M., Arakawa M., and Sakakibara R., “Effect of the fermented product cultivated from soybean milk using lactic acid bacteria, PS-B1, on liver function and lipid metabolism,” J. Integr. Stud. Diet. Habits., 22, 13-19, 2011.
[13] Sakakibara R., Kawatsu R., Honda H., Toda S., Yamaguchi K., and Nodake Y., “Effect of PS-B1 (a fermented product cultured from soybean milk using lactic acid bacteria) on hair growth in vivo. (1st report),” J Jpn Hair Sci Assoc., 51, 147-154, 2019.
[14] Fukasawa M., Nodake Y., Kawatsu R., Honda H., Yamaguchi K., and Sakakibara R., “In vivo efficacy evaluation of the fermented product cultivated from soybean milk using lactic acid bacteria, PS-B1, in STEMTM model of nonalcoholic steatohepatitis,” unpublished.
[15] Toda S., Nodake Y., and Sakakibara R., “Effect of PS-B1 (a fermented product cultured from soybean milk using lactic acid bacteria) on metabolic disturbances due to excessive sucrose intake,” Proceedings of The 3rd Int. Conf. on Pharma and Food, p. 124, 2016.
[16] Vogeser M., König D., Frey I., Predel H., Parhofer K. G., and Berg A., “Fasting serum insulin and the homeostasis model of insulin resistance (HOMA-IR) in the monitoring of lifestyle interventions in obese persons,” Clin. Biochem., 40, 964-968, 2007.
[17] Matthews D. R., Hosker J. P., Rudenski A. S., Naylor B. A., Treacher D. F., and Turner R. C., “Homeostasis model assessment: insulin resistance and β-cell function from fasting plasma glucose and insulin concentrations in man,” Diabetologia., 28, 412-419, 1985.
[18] Hosojima H., and Uchida K., “A practical clinical index of insulin resistance in obese patients with impaired glucose tolerance -An application of HOMA’s index-,” J. Japan Diab. Soc., 41, 433-441, 1998.
[19] Folch J., Lees M., and Sloane Stanley G. H., “A simple method for the isolation and purification of total lipides from animal tissues,” J. Biol. Chem., 226, 497-509, 1957.
[20] Larionov A., Krause A., and Miller W., “A standard curve based method for relative real time PCR data processing,” BMC Bioinform., 6, 2005, doi: 10.1186/1471-2105-6-62.
[21] Sawada T., Miyoshi H., Shimada K., Suzuki A., Okamatsu-Ogura Y., Perfield Ⅱ J. W., Kondo T., Nagai S., Shimizu C., Yoshioka N., Greenberg A. S., Kimura K., and Koike T., “Perilipin overexpression in white adipose tissue induces a brown fat-like phenotype,” PLoS One., 5, e14006, 2010, doi: 10.1371/journal.pone.0014006.
[22] Ma X., Hua J., and Li Z., “Probiotics improve high fat diet-induced hepatic steatosis and insulin resistance by increasing hepatic NKT cells,” J. Hepatol., 49, 821-830, 2008, doi: 10.1016/j.jhep.2008.05.025.
[23] Wang J., Tang H., Zhang C., Zhao Y., Derrien M., Rocher E., van-Hylckama Vlieg J. E. T., Strissel K., Zhao L., Obin M., and Shen J., “Modulation of gut microbiota during probiotic-mediated attenuation of metabolic syndrome in high fat diet-fed mice,” The ISME Journal., 9, 1-15, 2015.
[24] Kim B., Kwon J., Kim M-S., Park H., Ji Y., Holzapfel W., and Hyun C-K., “Protective effects of Bacillus probiotics against high-fat diet-induced metabolic disorders in mice,” PLoS One., 13, e0210120, 2018, doi: 10.1371/journal.pone.0210120.
[25] Ntambi J. M., Miyazaki M., Stoehr J. P., Lan H., Kendziorski C. M., Yandell B. S., Song Y., Cohen P., Friedman J. M., and Attie A. D., “Loss of stearoyl-CoA desaturase-1 function protects mice against adiposity,” Proc. Natl. Acad. Sci. U.S.A., 99, 11482-11486, 2002.
[26] Biddinger S. B., Almind K., Miyazaki M., Kokkotou E., Ntambi J. M., and Kahn C. R., “Effects of diet and genetic background on sterol regulatory element-binding protein-1c, stearoyl-CoA desaturase 1, and the development of the metabolic syndrome,” Diabetes., 54, 1314-1323, 2005.
[27] Rahman S. M., Dobrzyn A., Lee S-H., Dobrzyn P., Miyazaki M., and Ntambi J. M., “Stearoyl-CoA desaturase 1 deficiency increases insulin signaling and glycogen accumulation in brown adipose tissue,” Am. J. Physiol. Endocrinol. Metab., 288, E381-E387, 2005, doi: 10.1152/ajpendo.00314.2004.
[28] Gutiérrez-Juárez R., Pocai A., Mulas C., Ono H., Bhanot S., Monia B. P., and Rossetti L., “Critical role of stearoyl-CoA desaturase-1 (SCD1) in the onset of diet-induced hepatic insulin resistance,” J. Clin. Invest., 116, 1686-1695, 2006, doi: 10.1172/JCI26991.
[29] Xiao F., Deng J., Guo Y., Niu Y., Yuan F., Yu J., Chen S., and Guo F., “BTG1 ameliorates liver steatosis by decreasing stearoly-CoA desaturase 1 (SCD1) abundance and altering hepatic lipid metabolism,” Sci. Signal., 9, ra50, 1-11, 2016.
[30] Xiao F., Deng J., Yu J., Guo Y., Chen S., and Guo F., “A novel function of B-cell translocation gene 1 (BTG1) in the regulation of hepatic insulin sensitivity in mice via c-Jun,” The FASEB Journal., 30, 348-359, 2016.
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    Kyoshiro Yamaguchi, Marie Iwanaga-Suehiro, Kyoko Fujimoto, Masashi Fukasawa, Ryuzo Sakakibara. (2020). Improvement in High-fat Diet-induced Obesity and Insulin Resistance upon Uptake of PS-B1, a Fermented Product Prepared from Soy Flour Using Lactic Acid Bacteria. Journal of Food and Nutrition Sciences, 8(4), 81-88. https://doi.org/10.11648/j.jfns.20200804.12

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    ACS Style

    Kyoshiro Yamaguchi; Marie Iwanaga-Suehiro; Kyoko Fujimoto; Masashi Fukasawa; Ryuzo Sakakibara. Improvement in High-fat Diet-induced Obesity and Insulin Resistance upon Uptake of PS-B1, a Fermented Product Prepared from Soy Flour Using Lactic Acid Bacteria. J. Food Nutr. Sci. 2020, 8(4), 81-88. doi: 10.11648/j.jfns.20200804.12

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    Kyoshiro Yamaguchi, Marie Iwanaga-Suehiro, Kyoko Fujimoto, Masashi Fukasawa, Ryuzo Sakakibara. Improvement in High-fat Diet-induced Obesity and Insulin Resistance upon Uptake of PS-B1, a Fermented Product Prepared from Soy Flour Using Lactic Acid Bacteria. J Food Nutr Sci. 2020;8(4):81-88. doi: 10.11648/j.jfns.20200804.12

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  • @article{10.11648/j.jfns.20200804.12,
      author = {Kyoshiro Yamaguchi and Marie Iwanaga-Suehiro and Kyoko Fujimoto and Masashi Fukasawa and Ryuzo Sakakibara},
      title = {Improvement in High-fat Diet-induced Obesity and Insulin Resistance upon Uptake of PS-B1, a Fermented Product Prepared from Soy Flour Using Lactic Acid Bacteria},
      journal = {Journal of Food and Nutrition Sciences},
      volume = {8},
      number = {4},
      pages = {81-88},
      doi = {10.11648/j.jfns.20200804.12},
      url = {https://doi.org/10.11648/j.jfns.20200804.12},
      eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.jfns.20200804.12},
      abstract = {PS-B1 is a fermented product prepared from soy flour using lactic acid bacteria. Over a 10-week period, C57BL/6J mice were reared under laboratory conditions on a normal diet (control, n=5), high-fat diet (HF, n=5), or high-fat diet supplemented with 4% PS-B1 (HF-P, n=6). After 10 weeks, the change in weight gain, intestinal and epididymal fat accumulation, serum and liver biochemical parameters, and gene expression in the mice was investigated. HF diet-induced weight gain and increase in intestinal and epididymal fat accumulation were lower in mice fed with HF-P diet than in mice fed with HF diet, suggesting that PS-B1 prevented HF diet-induced obesity in HF-P mice. Furthermore, the levels of liver lipids (triglycerides, TG; non-esterified fatty acid, NEFA; total cholesterol, TC), serum TC, serum glucose, and serum insulin were significantly increased in the HF group than those in control mice. In HF-P mice, neither serum TC nor serum glucose levels were reduced. In contrast, the levels of liver lipids and serum insulin were lower in HF-P mice than in HF mice, suggesting that PS-B1 reduced these parameters in HF-P mice. The homeostatic model assessment of insulin resistance (HOMA-IR) value, which was calculated from the serum glucose and insulin levels, was 21.5 ± 4.2 in the HF mice. However, the HOMA-IR (8.2 ± 0.2) values were significantly decreased in the HF-P mice, suggesting that PS-B1 improves insulin resistance. Additionally, we compared the expression levels of stearoyl-CoA desaturase-1 (Scd1) in the liver. Quantitative RT-PCR showed increased expression of Scd1 in HF mice compared to that in control mice. Furthermore, ingestion of PS-B1 led to reduced expression of Scd1 mRNA in HF-P mice, implying that PS-B1 is effective in reducing the expression of the gene encoding SCD1. These results suggest that the anti-obesity effect of PS-B1 and improvement in fat accumulation upon PS-B1 uptake may be due to improvement in insulin resistance and reduction in the expression level of Scd1.},
     year = {2020}
    }
    

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  • TY  - JOUR
    T1  - Improvement in High-fat Diet-induced Obesity and Insulin Resistance upon Uptake of PS-B1, a Fermented Product Prepared from Soy Flour Using Lactic Acid Bacteria
    AU  - Kyoshiro Yamaguchi
    AU  - Marie Iwanaga-Suehiro
    AU  - Kyoko Fujimoto
    AU  - Masashi Fukasawa
    AU  - Ryuzo Sakakibara
    Y1  - 2020/07/04
    PY  - 2020
    N1  - https://doi.org/10.11648/j.jfns.20200804.12
    DO  - 10.11648/j.jfns.20200804.12
    T2  - Journal of Food and Nutrition Sciences
    JF  - Journal of Food and Nutrition Sciences
    JO  - Journal of Food and Nutrition Sciences
    SP  - 81
    EP  - 88
    PB  - Science Publishing Group
    SN  - 2330-7293
    UR  - https://doi.org/10.11648/j.jfns.20200804.12
    AB  - PS-B1 is a fermented product prepared from soy flour using lactic acid bacteria. Over a 10-week period, C57BL/6J mice were reared under laboratory conditions on a normal diet (control, n=5), high-fat diet (HF, n=5), or high-fat diet supplemented with 4% PS-B1 (HF-P, n=6). After 10 weeks, the change in weight gain, intestinal and epididymal fat accumulation, serum and liver biochemical parameters, and gene expression in the mice was investigated. HF diet-induced weight gain and increase in intestinal and epididymal fat accumulation were lower in mice fed with HF-P diet than in mice fed with HF diet, suggesting that PS-B1 prevented HF diet-induced obesity in HF-P mice. Furthermore, the levels of liver lipids (triglycerides, TG; non-esterified fatty acid, NEFA; total cholesterol, TC), serum TC, serum glucose, and serum insulin were significantly increased in the HF group than those in control mice. In HF-P mice, neither serum TC nor serum glucose levels were reduced. In contrast, the levels of liver lipids and serum insulin were lower in HF-P mice than in HF mice, suggesting that PS-B1 reduced these parameters in HF-P mice. The homeostatic model assessment of insulin resistance (HOMA-IR) value, which was calculated from the serum glucose and insulin levels, was 21.5 ± 4.2 in the HF mice. However, the HOMA-IR (8.2 ± 0.2) values were significantly decreased in the HF-P mice, suggesting that PS-B1 improves insulin resistance. Additionally, we compared the expression levels of stearoyl-CoA desaturase-1 (Scd1) in the liver. Quantitative RT-PCR showed increased expression of Scd1 in HF mice compared to that in control mice. Furthermore, ingestion of PS-B1 led to reduced expression of Scd1 mRNA in HF-P mice, implying that PS-B1 is effective in reducing the expression of the gene encoding SCD1. These results suggest that the anti-obesity effect of PS-B1 and improvement in fat accumulation upon PS-B1 uptake may be due to improvement in insulin resistance and reduction in the expression level of Scd1.
    VL  - 8
    IS  - 4
    ER  - 

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Author Information
  • Department of Biochemistry, Faculty of Pharmaceutical Sciences, Nagasaki International University, Sasebo, Nagasaki, Japan

  • Department of Biochemistry, Faculty of Pharmaceutical Sciences, Nagasaki International University, Sasebo, Nagasaki, Japan

  • Department of Biochemistry, Faculty of Pharmaceutical Sciences, Nagasaki International University, Sasebo, Nagasaki, Japan

  • Department of Biochemistry, Faculty of Pharmaceutical Sciences, Nagasaki International University, Sasebo, Nagasaki, Japan

  • Department of Biochemistry, Faculty of Pharmaceutical Sciences, Nagasaki International University, Sasebo, Nagasaki, Japan

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